杭白菊中主要黄酮苷的测定及影响因素考察

Determination of main flavone glycosides in Flos Chrysanthemi and observation of factors influenced contents

目的:测定杭白菊中木犀草素-7-O-β-D-葡萄糖苷和芹菜素-7-O--βD-葡萄糖苷含量,并考察影响其含量的因素。方法:采用反相高效液相色谱法,以Zorbax SB C18为分离柱,乙腈-10 mmol.L-1磷酸盐缓冲液(pH 2)为流动相进行梯度洗脱,检测波长338 nm。结果:不同种植基地、不同采摘时间、不同炮制工艺及不同等级的杭白菊中木犀草素-7-O--βD-葡萄糖苷和芹菜素-7-O-β-D-葡萄糖苷的含量有一定的变化;不同年度的杭白菊中2成分含量差别不大;不同产地及不同品种菊花2成分含量差异较大。结论:杭白菊2成分的含量与种植基地、炮制工艺等有一定的关系,产地、品种对其含量的影响更大。

Objective： To determine and compare the content of luteolin-7-O-β-D-glucoside and apigenin-7-O-β-D-glucoside in Flos Chrysanthemi from different collection time, sources, grades and processes. Method： The contents were determined by RPHPLC. Zorbax SB C18 column （4. 6 mm ×250 mm, 5 μm） was used as analysis column, the mobile phase was acetonitrile-pH 2. 0 phosphate buffer solution with gradient elution, the detector was set at 338nm. Result： The contents of two components changed at some degree in Flos Chrysanthemi from different collection time, different plant sites or with different grades, while the contents varied obviously among Flos Chrysanthemi from different source and different sorts. No obvious difference was found in Flos Chrysanthemi from different year. Conclusion： The contents of two components were influenced by process, plane site, source and sorts, especially by source and sorts.