摘要
本文采用高效液相色谱内标法,以乙腈+水为流动相,邻苯二甲酸二甲酯为内标物,使用SymmetryShieldTMRP-C18不锈钢柱和二极管阵列检测器,在237nm波长下对试样中的烟嘧磺隆进行分离和定量分析。结果表明烟嘧磺隆的线性关系系数为0.9998,测定4%烟嘧磺隆悬浮剂时,标准偏差为0.029,变异系数为0.70%,平均回收率为99.92%;测定烟嘧磺隆原药时,标准偏差为0.23,变异系数为0.26%,平均回收率为100.16%。
Quantitative analysis of nicosulfuron was conducted by HPLC using an Symmetry Shield^TM RP-C18 column, acetonitrile＋water as mobile phase, dimethyl-o-phthalate as internal standard and DAD at 237nm. The results showed that the linear correlation coefficient was 0.999 8, the standard deviation was 0.029, the coefficient of variation was 0.70%, the average recovery was 99.92% when nicosulfuron 4% SE was determined; the standard deviation was 0.23, the coefficient of variation was 0.26%, the average recovery was 100.16% when nicosulfuron TC was determined.
出处
《农药科学与管理》
CAS
2006年第10期6-9,共4页
Pesticide Science and Administration
