牡丹皮高效液相色谱指纹图谱研究

Studies on HPLC fingerprint of Cortex Moutan

目的:建立牡丹皮的高效液相色谱指纹图谱,为科学评价及有效控制其质量提供可靠方法。方法:利用HPLC-DAD,梯度洗脱,测定了30批牡丹皮药材样品,并应用LC-MS和MS-MS技术指认指纹图谱中的主要色谱峰。结果:30批牡丹皮样品得到的色谱指纹图谱有15个共有峰,指认了色谱峰的化学归属,主要特征峰的峰面积排序依次为:丹皮酚>五没食子酰葡萄糖>没食子酸甲酯>没食子酰芍药苷>没食子酸>羟基芍药苷>其他色谱峰。结论:牡丹皮的指纹图谱特征性及专属性强,可结合含量测定用于全面控制牡丹皮的质量。

Objective： To establish a HPLC fingerprint for quality evaluation of Cortex Moutan. Method： The HPLC chromatographic fingerprinting of 30 lots of Cortex Moutan was established and major peaks were identified by LC-MS and MS-MS. Result： The HPLC fingerprint of Cortex Moutan was established, showing 15 characteristic peaks. The areas of these peaks were found to complied with the following rule： paeonol 〉 1,2, 3,4, 6-penta-O-galloyl-glucos 〉 methyl gallate 〉 galloylpaeoniflorin 〉 gallic acid 〉 oxypaeoniflorin 〉 other compounds. Conclusion： The chromatographic fingerprinting of Cortex Moutan with high specificity can be used to control its quality and monitor lot to lot consistency.