杏香兔耳风组培快繁技术

Tissue Cultural Techniques in Ainsliaea fragrans

以杏香兔耳风根颈以上部位作为外植体,采用组织培养技术,对其进行芽的诱导、丛生芽增殖、试管苗生根及试管苗野外移栽技术等作了较系统研究。结果表明:基本培养基MS附加6-BA2.0mg/L(单位下同)+NAA0.5的培养基上进行芽的诱导,在6-BA1.0+NAA0.5的培养基上进行增殖培养,并在1/2MS+NAA0.3的培养基中进行生根培养,是较理想的组培再生条件,增殖系数为5,生根率达99.8%,平均每株苗木的根系数量达10以上,组培苗移栽成活率达96.6%。

There are tested on the buds inducing, the buds regenerating and the shoots rooting of Ainsliaeafragrans. The results demonstrated that the best medium for culture is MS＋6-BA2.0mg/L （the unit as same as follows）＋NAA0.5, MS＋6-BA1.0＋NAA0.5 and 1/2MS＋ NAA0.3, respectively. And the coefficient of regenerating is about 5, the rooting rate reached 99.8%, and the amount of root is above 10. Meanwhile, the survival rate for tissue etdtural seedling transplanting is 96.6%.