摘要
采用超声波+复合酶法预处理对Phellinuslinteus子实体水溶多糖进行提取,并对提取多糖成分差异进行初步分析。采用超声波技术预处理桑黄子实体,利用正交实验研究了料液比、时间、功率各因素对桑黄多糖得率的影响;在超声波优化结果基础上,进行纤维素酶+木瓜蛋白酶法处理,研究了纤维素酶用量、处理时间、木瓜蛋白酶用量、处理时间各因素对多糖得率的影响。研究表明:在料液比1:20、超声波(400W、20min)和纤维素酶(0.8%、pH5.5、45℃、60min)、木瓜蛋白酶(2%、pH5.5、50℃、120min)双重预处理条件下,再进行多糖提取,多糖得率最高。研究表明采用超声波+复合酶法预处理方法提取多糖,与传统的热水提取法相比,极大提高了提取效率,为Phellinuslinteus多糖的产业化打下了基础。
This paper is to study optimization extraction of polysaccharide from Phellinus linteus using sonication and compound enzyme hydrolysis method. Firstly, we optimized the ratio of stuff and fluid,extraction time, power by the orthogonal design in sonication process. Following this, the effect of concentration, treating time of cellulose and papain on extraction yield of polysaccharide was studied respectively. The result show that the optimization condition is Phellinus linteus： water （ 1：20, w：v）, ultrasonic （400 W,20 min）, cellulose（0.8 %, pH 5.5, 45 ℃ ,60 min）, papain （2 %, pH 5.5,50 ℃, 120 min）. Compared with the traditional water extraction method, the ultrasonic ＋ compound enzyme extraction method can improve the polysaccharide yield, which can be used in industrial process of Phellinus linteus polysaccharide.
出处
《食品研究与开发》
CAS
北大核心
2006年第10期32-35,共4页
Food Research and Development
