流式细胞术分析膀胱移行细胞癌中血型抗原Lewis X的表达

Expression of Lewis X in bladder transitional cell carcinoma by flow cytometry analysis

目的探讨血型抗原LewisX在膀胱移行细胞癌中的表达及其意义。方法应用流式细胞技术(FCM)对膀胱移行细胞癌组织和非肿瘤膀胱黏膜组织标本进行LewisX抗原的测定,结果用阳性细胞百分率表示,并结合临床资料分析LewisX抗原在膀胱移行细胞癌中的作用。结果LewisX抗原在膀胱移行细胞癌中的阳性表达为(52.58±22.69)%,在非肿瘤膀胱组织中的阳性表达为(24.38±4.97)%,两者间有显著性差异(P<0.01);LewisX的阳性表达在膀胱移行细胞癌的临床分期,病理分级中差异无统计学意义(P>0.05)。结论LewisX在膀胱癌中高表达对膀胱癌的诊断有一定的帮助,FCM可作为检测LewisX的一种快捷方法。

Objective To study the expression of Lewis X antigen in transitional cell carcinoma of bladder. Methods Flow cytometry was applied to examine Lewis X antigen positive cells in bladder transitional cell carcinoma and normal mucosa. Results The percentage of Lewis X antigen positive cells was （52.58±22.69）% in cancer tissue and （24.38 ± 4.97）% in normal tissue（P〈0.01）. The Expression of Lewis X showed no relation to the grade and stage of bladder transitional cell carcinoma（P〉0.05）. Conclusion Lewis X antigen can be helpful in the detection of bladder transitional cell carcinoma. Flow cytometry to detect Lewis X antigen can be an effective way.