T-DNA插入产生的水稻多分蘖突变的遗传分析

Genetic Analysis of a More-Tiller Mutation by T-DNA Insertion in Rice(Oryza sativa L.)

在筛选和鉴定水稻T-DNA(含Basta抗性基因Bar)插入纯合体的过程中,观察到一个多分蘖的突变体。其分离群体中多分蘖和正常分蘖两种植株类型的分离比率为3∶1,符合1对基因的显性遗传。Basta抗性检测、PCR分子检测及Southern杂交证实,该突变体是由单一T-DNA插入引起的,多分蘖性状与T-DNA共分离。该突变材料可用于插入座位的基因克隆。

T-DNA tagging has been used to isolate genes in higher plant. In this study, a more-tiller mutant caused by T- DNA insertion in rice was identified. Genetic analysis of the mutant showed that the two types of phenotype, more-tiller and normal tiller in the segregation populations derived from the T-DNA heterozygotes fit the ratio of 3： 1. Test for Basra resistance showed the more-tiller plants were all resistant while the normal tiller plants were susceptible, and the ratio of resistant and susceptible plants was 3：1, which indicated that the more-tiller mutant was co-segregated with Basra resistance. The more-tiller mutant caused by T-DNA insertion was confirmed by T-DNA detection using PCR method and Southern Blot. This more-tiller mutant is useful for isolation of the tagged gene in rice.