薄层扫描法测定贝芪口服液中黄芪甲甙的含量

DETERMINATION OF ASTRAGALOSIDE IN BEIQI ORAL LIQUID BY TLCS

建立了测定贝芪口服液中黄芪甲甙含量的薄层扫描测定法。采用硅胶Ｇ－ＣＭＣＮａ薄层板，以氯仿－甲醇－水（６５：３５：１０）为展开剂、１０％硫酸乙醇为显色剂，测定波长（λ_s）与参比波长（λ_Ｒ）分别为６００、７００ｎｍ。黄芪甲甙的量在１～６μｇ与斑点面积呈线性关系，ｒ＝０．９９８８。方法平均回收率为９８．２６±２．７６％（ｎ＝６）．三批供试品中黄芪甲甙的含量为０．１２２～０．１８０ｍｇ／ｍｌ，方法精密度（ＲＳＤ）分别为３．１０％、３．７１％、３．７０（ｎ＝４）。

The determination of astragaloside in Beiqi oral liquid by TLCS was reported in this paper.The samples were extracted by water-saturated n-butanol.The-butanol layer was separated and washed by n-butanol-saturated water. The astragaloside in extracts was separated on silica gel G-0.5% CMC Na plate by using chloroform-methanol-water (65:35:10) as developing solvent and using 1%sulfuric acid in alcohol as location reagent. The detection wavelen6ths were 600nm(λs)and 700nm(λR).The liner range of this method was 1～6mg/ml(r= 0.9988)for astragaloside. The average recovery was 98. 26±2.76%(n =6).The precisions of analytical method were 3. 10%、3.71%and3.70%(n=4 )in three samples respectively. The results for the determination of astragaloside in three batches of Beiqi oral liquid were 0.117～0. 189% mg/ml.The other ingredients from oral liquid do not interfere with astragaloside determination.The method can be used for quality control of this preparation.