摘要
该研究以培养人体血管平滑肌细胞(VSMC)为模型,用3H-TdR掺入试验观察钙拮抗剂(Nifedipine、Amlodipine)和ACEI(Cilazapril、Benazapril)对细胞增殖效应。结果3种浓度钙拮抗剂都明显抑制细胞增殖(P<0.01),高浓度(10-5mol/L、5×10-6mol/L)时Nifedipine抑制程度更好(P<0.05),低浓度(10-6mol/L)以Amlodipine为佳(P<0.05)。ACEI中发现高浓度时2种药物均能抑制细胞增殖(P<0.01),而低浓度时细胞生长反常地超过对照组(P<0.05)。钙拮抗剂与ACEI比较,前者尤其是Nifedipine抑制细胞增殖程度较ACEI明显。由此推测小剂量ACEI反而刺激细胞增生是否与药物含量较低时仅抑制血浆ACE活性,不能抑制组织ACE活性有关。
Proliferation of vascular smooth muscle cells(VSMCs)plays a key role in the evolution of restenosis after coronary angioplasty. An observation was made on the effect of calcium antagonists(Nifedipine,Amlodipine) and ACEIs(Cilazapril, Benazapril)on cultured human VSMCs using 3H-TdR incorporation as parameter for growth. Our resulte showed that all three different concentrations of the calcium antagonists inhibited VSMC growth significantly(P< 0.01);high concentration Nifedipine yielded a better result;while at low concentration, Amlodipine seemed to show a greater inhibition。 As to ACEIs both Cilazapril and Benazapril in high concentration inhibited growth of VSMCs;however at low concentration VSMCs growth paradoxically increased compared to control.Between calcium antagoniste and ACEls, the former showed a greater inhibition. It was postulated that low concentration of ACEIs was not able to inhibit VSMC growth,A search for potent tissue ACEIs was not able to inhibit VSMC growth,A search for potent tissue ACEIs may be the solution for prevention of restenosis after coronary angioplasty。
出处
《上海第二医科大学学报》
CSCD
1996年第5期308-310,共3页
Acta Universitatis Medicinalis Secondae Shanghai
关键词
血管紧张素
转换酶抑制剂
钙拮抗剂
平滑肌细胞
angiotensin converting enzyme inhibitor
calcium antagonist
vascular smooth muscle cells
proliferation