土贝母皂苷体外抗乙型肝炎病毒的药效研究

Effect of Tubeimoside in Anti-HBV Activity in vitro

目的观察土贝母皂苷体外抗乙型肝炎病毒(HBV)效果。方法以2.2.15细胞为靶细胞,给药后通过检测细胞表面及培养液中HBsAg,HBeAg和HBV-DNA,观察土贝母皂苷抗HBV效果。结果土贝母皂苷各浓度组对HBsAg,HBeAg表达均有抑制作用,但以0.1 mg/m l作用48 h时最强,分别为43.46%和54.52%(P<0.01);对HBV-DNA的抑制作用以1 mg/m l组显著(P<0.05)。结论土贝母皂苷在体外有抗乙型肝炎病毒(HBV)作用。

Objective To study the effect of anti-HBV activity in vitro. Methods The anti-HBV activity of Tubeimoside was ob- served in 2.2.15cells （human hepatocellular carcinoma cells stably transfected with HBV）. Results Tubeimoside could significantly reduce HBsAg,HBeAg level. After 48 hours of continuous exposure to Tubeimoside at the concentration of 0.1 mg/ml in vitro culture, the inhibitions of HBsAg,HBeAg were 43.46% ,54.52% respectively（P 〈 0.01 ）. While the inhibitions of HBV DNA is significant at the concentration of 1mg/ml in vitro culture. Conclusion The anti-HBV effect of Tubeimoside is demonstrated in 2.2.15 cells.