活络镇痛片中欧前胡素含量的测定

Determination of Imperatorin in Huoluo Zhentong Tablet by HPLC

目的建立活络镇痛片中欧前胡素的含量测定方法。方法采用HPLC,ODS-Hypersil色谱柱(4.6 mm×200 mm,5μm);乙腈∶水(45∶55)为流动相;检测波长249 nm。结果平均回收率为98.4%,RSD=2.53%。结论所建方法准确,可靠,可用于该药的质量控制。

Objective To establish an HPLC method for determination of imperatorin in Huoluo Zhentong Tabeles. Methods ODS-Hypersil column（4.6 mm × 200 mm,5μm） was used; The mobile phase was aeetonitrile-water（45：55）, and detection wavelength was 249 nm. Results The average rate of recovery was 98.4%, RSD = 2.53%. Conclusion The method is accurate and reliable. It can be used for determination of imperatorin in Huoluo Zhentong Tablets.