苦参药材中4种生物碱的含量测定

Determination of 4 Kinds of Alkaloids in Sophora flavescens Ait.by HPLC

目的建立反相液相色谱法测定苦参药材中4种生物碱的含量测定方法。方法使用hypersil BDS（150mm×4．6mm，5μm）色谱柱，流动相为甲醇-0．1％氨水梯度洗脱，检测波长210nm。结果苦参碱在0．25～2．00μg（r=0．9997），槐定碱在0．28～2．24μg（r=0．9995），氧化苦参碱在0．244-1．953μg（r=0．9991），氧化槐果碱在0．262-2．096μg（r=0．9992）范围呈良好的线性关系，回归方程分别为y=2602．6x-72．031，y=1558．8x＋17．381，y=1755．8x＋162．59，y=2219．55x-21．119，加样回收率分别为99．24％，98．31％，101．28％，100．59％，RSD分别为1．248％，1．517％，1．412％，1．638％。结论该法简便，快速，专属性好，可用于苦参药材中生物碱的含量测定。

Objective To develop an HPLC method to determine the content of 4 alkaloids in Sophora flavescens Ait. Methods Hypersil BDS（ 150 mm × 4.6 mm,5 um）phase column was used with mobile phase methanol- 0.1% ammonia water gradient elution ,the wavelength of determination was 210 nm. Results The linear concentration range of matrine ,sophoridine,oxymatrine and oxysophocarpine were 0.25 - 2.00 μg（ r = 0.999 7 ） ,0.28 - 2. 24μg （ r = 0. 999 5 ） ,0. 244 - 1. 953 μg （ r = 0. 999 1 ） ,0. 262 - 2. 096μg（ r = 0. 999 2 ）, regression equation . were ：y = 2 602.6x - 72.031 ,y = 1 558.8x ＋ 17.381 ,y = 1 755.8x ＋ 162.59 ,y = 2219.55x - 21.119, average recovery were 99.24% ,98.31% , 101.28% , 100.59% ,RSD were 1. 248% , 1. 517%, 1. 412%, 1. 638%, respectively. Conclusion This method is simple,rapid, sensitive, specific, and can be used for determination of the content of alkaloids in Sophoraflavescens Ait .