反相高效液相法测定滋肾利肝颗粒中芍药苷的含量(英文)

Determination of the Content of Paeoniflorin in Zishenligan Granule by Reversed-Phase High Performance Liquid Chromatography

目的建立一种测定滋肾利肝颗粒中芍药苷含量的反相高效液相法。方法采用Kromasil C18柱，以甲醇-0．05mol·L^-1磷酸二氢钾溶液（32：68）为流动相，紫外检测波长230nm，流速1ml·min^-1。结果在所使用的色谱条件下，芍药苷出峰位置未见干扰，芍药苷在24．28～121．40μg·ml^-1浓度范围内线性关系良好（r=0．9999）。滋肾利肝颗粒中芍药苷的平均加样回收率为98．32％，相对标准偏差为1．34％（n=9）。结论该法操作简便，重现性好，可作为滋肾利肝颗粒生产上质量控制的一种可信工具。

Objective To establish a RP - HPLC method for determination of the content of Paeoniflorin in Zishenligan Granule. Methods The analysis was carried out by using a Kromasil C18 analytical column with methanol and 0.05mol·L^-1 potassium dihydrogen phosphate solution （32：68, v/v） as mobile phase. The UV detection wavelength was 230nm and the flow rate was 1ml·min^-1 Results Under the chromatographic conditions used, there was no interference found in the position of Paeoniflorin peak. Paeoniflorin had fine linear responses （ r = 0. 999 9） in the concentration range of 24.28 - 121.40 μg·ml^-1, the average recovery of Paeoniflorin in Zishenligan Granule was 98.32% with the relative standard deviation of 1.34% （ n = 9）. Conclusion The method employed in this analysis is convenient with good repetition and can be used as a reliable tool for quality control in the production of Zishenligan Granule.