急性白血病患者SHP-1与c-kit基因表达及其临床意义

Expression and Clinical Value of SHP-1 and c-kit in Acute Leukemia

为了探讨造血细胞磷酸酶(SHP-1)基因和原癌基因c-kit在急性白血病(AL)患者中的表达及其与AL的发生及预后的关系,应用半定量逆转录-聚合酶链反应(RT-PCR)方法检测60例急性白血病患者及33名正常对照者单个核细胞的SHP-1、c-kitmRNA的表达。结果显示:SHP-1在AML细胞中表达阳性率由高至低依次为缓解组、初治组、复发组,c-kit表达阳性率则相反,各组与正常对照组相比有显著性差异(P<0.05),SHP-1在AML细胞中表达阳性率比ALL细胞高,有显著性差异(P<0.05),c-kit在AML细胞中表达阳性率比ALL细胞中高,有显著性差异(P<0.05);SHP-1与c-kit表达成负相关(r=-0.502,P<0.05);30例初治AML患者SHP-1+与SHP-1-的完全缓解率均有显著性差异(P<0.05),c-kit+与c-kit-的完全缓解率均有显著性差异(P<0.05)。结论:SHP-1可能是一个潜在的抑癌基因,它负性调节c-kit基因而抑制肿瘤的发生,同时监测二者的表达可作为判断AL治疗转归的预测指标。

The aim of study is to investigate the expression of hematopoietic cell phosphatase （ SHP-1 ） gene and c-kit pro-oncogene in acute leukemia（AL） and its impact on prognosis in AL. Semi-quantity reverse transcriptase-polymerase chain reaction（RT-PCR） was used to detect the expression of SHP-1 mRNA and c-kit mRNA in 60 AL patients and 33 normal controls （NC）. The results showed that the positive rates of SHP-1 expression from high to low level were found orderly in complete remission group, newly diagnosed group and relapsed group, there was significance difference between each group and NC group（ P 〈 0.05 ）. The positive rates of c-kit expression were opposite order in each groups as compared with SHP-1. there was also significance difference between each group and NC group（ P 〈 0.05 ）. The positive rate of SHP-1 and c-kit expressions in AML was higher than that in ALL（ P 〈 0.05 ）, there was nagative correlation between expressions of SHP-1 and c-kit （ r = -0. 502,P 〈0.05） ; The difference between the complete remission rate in SHP-1 positive and in SHP-1 negative patients from 30 newly diagnosed AML patients was significant （ P 〈 0. 05 ）, the same result was found between c-kit ^＋ complete remission and c-kit- complete remission. It is concluded that SHP-1 gene is a potentially anti-oncogene and inhibits the growing of tumor by negatively modulating c-kit gene. Simultaneous detection of SHP-1 and c-kit gene may act as a factor for predicting prognosis in AL.