原发性肝细胞癌p33^(ING1b)表达水平及其临床诊断意义

Expression level and clinical significance of p33^(ING1b) in primary hepatocellular carcinoma

目的探讨肝癌p33ING1bmRNA表达水平及与临床病理特征的关系,揭示p33ING1b基因在肝癌发生发展中的作用及临床诊断意义。方法用逆转录聚合酶链反应(RT-PCR)检测55例原发性肝癌患者癌组织及其癌旁组织p33ING1bmRNA水平。结果63·6%(35/55)癌组织及70·9%(39/55)癌旁组织p33ING1bmRNA呈阳性表达,癌组织p33ING1bmRNA表达量为0·410±0·175,明显低于癌旁组织0·529±0·203,两者比较差异有统计学意义(P<0·05)。p33ING1bmRNA水平与肝癌的病理分级、淋巴结转移、包膜浸润与否有关,而与年龄、性别、肿瘤大小、癌栓形成、合并肝硬化与否无关。结论p33ING1b表达下调在肝癌发生、发展中起重要作用,可以作为肝癌分化、侵袭性及预后的评价指标。

Objective To investigate the p33^ING1b mRNA level and the relationship to clinical pathological data in primary hepatocellular carcinoma （PHC）. Methods Reverse transcriptase-polymerase chain reaction （RT-PCR） method was employed to detect the mRNA level of p33^ING1b in liver cancer tissues and adjacent tissues of cancer. Results p33^ING1b mRNA was positive expression in 63. 6% （35/55） cancer tissues and 70. 9% （39/55） adjacent tissues of cancer. The expression level of p33^ING1b mRNA in cancer tissues （0. 41±0 0. 175） was significantly lower than that in adjacent tissues of cancer （0. 529 ±0. 203）. The level of p33^ING1b was significantly associated with pathological grades, lymph node metastasis and envelope infiltration, but not associated with sex, age, tumor size, tumor thrombus or liver cirrhosis. Conclusion The decrease of p33^ING1b may play an important role in the tumorigenesis and development of PHC, and may be an adjuvant parameter in evaluating tumor differentiation, invasion and prognosis.