血清IFN-γ和IL-4在急性白血病中表达的研究

Study on interferon-γ and interleukin-4 levels in acute leukemia patients

目的研究急性白血病患者初治时和完全缓解后免疫功能的变化,为有效的免疫治疗提供依据。方法用ELISA方法检测IFN-γ的水平,用放免方法检测IL-4的水平。结果急性白血病初治组及短期CR组IFN-γ水平分别为(17.32±10.68)pg/mL,(22.63±8.98)pg/mL明显低于长期CR组(48.90±9.84)pg/mL及正常对照组(51.4±10.87)pg/mL,初治组及短期CR组IL-4水平分别为(3.26±0.49)ng/mL,(2.96±0.41)ng/mL明显高于长期CR组(1.09±0.45)ng/mL及正常对照组(0.78±0.49)ng/mL。初治组IFN-γ和IL-4水平与长期CR组及正常对照组比较,差异有显著性,P<0.01。结论初治及短期CR急性白血病患者体内以Th2型细胞因子占优势,造成免疫抑制状态,与IFN-γ、IL-4水平有关,白血病易于复发。随着CR时间的延长免疫状态逐渐恢复。IFN-γ、IL-4的水平可作为临床上评估患者免疫状态和预后的指标。

[Objective] To study the change of immune function of serum IFN-γ and IL--4 of acute leukemia patients in fast visit and CR stage, and provide the theoretical foundation for effective immunotherapy. [Methods] Level of IFN-γ was measured by ELISA, and level of IL-4 was measured by immunology. [Results] Compared with long stage CR patients [（48.90±9.84） pg/mL, （1.09±0.45） ng/mL] and healthy controls [（51.4±10.87） pg/mL, （0.78±0.49） ng/mL], the IFN-γ levels decreased significantly and the IL-4 levels increased significantly in first visits [（17.32±10.68 pg/mL, （3.26±0.49） ng/mL] and short stage CR patients [（22.63±8.98） pg/mL, （2.96±0.41） ng/mL]. Compared with long stage CR patients and healthy controls, there were significant in first visit patients （P 〈0.01）. [Conclusion] Th2 type cytokines were dominant in the first visit and short stage CR acute leukemia patients and contributed to the immunosuppressive state, leukemia was easy to recur and related to the levels of IFN-γ and IL- 4. Along with time extent in CR stage, the immunological function recoved gradually. Therefore we could estimate immune function of the acute leukemia patients and prognosis by assaying the levels of IFN-γ and IL-4.