毒蕈碱样乙酰胆碱受体m1～m5亚基在大鼠前庭终器组织中的表达

Expression of mACh receptor m1-m5 subunits in the vestibular endorgans of rat

目的：检测大鼠前庭终器中毒蕈碱样乙酰胆碱受体（mAChRs）m1～m5哑基的表达。方法：根据GeneBank公布的大鼠mAChR m1～m5亚基的mRNA序列设计特异性引物，再运用RTPCR方法检测大鼠前庭终器中编码mAChR m1～m5亚基的mRNA的表达，之后行PCR产物直接测序。以大鼠脑千组织提取RNA为阳性对照，以解剖液为阴性对照。结果：扩增出标志mAChR m1～m5亚基表达的PCR产物，并经测序证实与预计扩增的cDNA片段序列一致。结论：在大鼠前庭终器组织中有mAChR m1～m5亚基的表达，为乙酰胆碱作为大鼠外周前庭系统冲经递质提供了最直接的证据。

Objective：To investigate the expression of mA（Th recet）tor m1-m5 subunits in the vestibular endorgans of rat. Method：Gene specific primers used were based upon the rat mACh receptor m1—m5 subunits sequence. Reverse transcription polymerase chain reaction （RT-PCR） was carried out on the vestibular endorgans. The PCR products were directly sequenced. Result： It was shown that roACh receptor m1-m5 subunits mRNA was detected and sequenced from vestibular endorgans by RT-PCR technique. Sequence analysis of the PCR products confirmed identity to rat mACh receptor m1—m5 subunits cDNA sequence in the predicted region. Conclusion： Present data confirmed the expression of ACh receptor m1—m5 subunits in the vestibular endorgans of rat. giving the direct evidence to date that ACh is one of the important neurotransmitters in the peripheral vestibular system of rat.