血管内皮生长因子936T/C基因多态性与结直肠癌及术后吻合口瘘的关系

Association between vascular endothelial growth factor gene 936 T/C polymorphism and colorectal cancer together with anastomotic leakage

目的通过研究血管内皮生长因子(VEGF)936T/C基因多态性了解该因子对结直肠癌生成及术后结肠吻合口瘘的影响。方法采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法检测结直肠癌及正常对照的VEGF936T/C基因型。结果结直肠癌患者VEGF936T/C基因型或等位基因与对照组相比无差异。伴有术后结肠吻合口瘘的结直肠癌患者其VEGF936C/C基因型及C等位基因比例低于无术后结肠吻合口瘘的患者,两者差异有统计学意义(P<0·05)。结论VEGF936C/C基因型或936C等位基因与结直肠癌的发生无关,但提示术后结肠吻合口瘘的发生率降低。

Objective To investigate the association between VEGF gene 936 T/C polymorphism and colorectal cancer together with anastomotic leakage. Methods Polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） was used to detect the VEGF 936 T/C genotypes in colorectal cancer patients and healthy controls. Results There was no significant difference in the frequency of VEGF 936 C/C genotype or C allele between colorectal cancer patients and healthy controls （ P 〉 0. 05 ）. The C/C genotype or C allele in colorectal cancer patients with anastomotic leakage was less frequently found than in the group without anastomotic leakage （ P 〈 0.05 ）. Conclusions VEGF 936 C/C genotype or C allele is not related to the development of colorectal cancer, but they can reduce the risk of anastomotic leakage after surgery in colorectal cancer patients.