精制清开灵干预MCAO再灌注损伤的药效及机制研究

Mechanism of therapeutic effect of QingKaiLing-ingredient combination on focal cerebral ischemia-reperfusion injury

目的:观察工艺改进后的精制清开灵对大鼠局灶脑缺血再灌注损伤的拮抗作用。方法:选取成年雄性大鼠120只,采用线栓法建立大鼠脑缺血再灌注模型,应用核磁共振弥散加权成像技术、病理学指标判断不同治疗组对大鼠脑缺血再灌注的拮抗作用,原位杂交技术检测脑组织海马脑源性生长因子(BDNF)含量的变化。结果:精制清开灵减轻脑缺血大鼠再灌注损伤,核磁共振表现为病灶周边区RA值,FA值明显高于模型组,脑源性生长因子BDNF表达多于模型组。结论:精制清开灵具有脑神经保护作用,在有效时间窗内给予该药物的干预可能是减少脑缺血再灌注损伤的重要措施,其作用与BDNF有关。

AIM： To elucidate the therapeutic effect and mechanism of QingKaiLing （QKL） - ingredient combination on cerebral ischemia. METHODS： Rat focal brain ischemia reperfusion injury was produced by occlusion in the middle cerebral artery, MRI and histological, analysis were used to evaluate the brain injury induced by iscbemia and the attenuations by the drugs including QKL - ingredient combination and the positive control nimodipine. In situ hybridization was used to measure the expression of BDNF mRNA in the brain. RESULTS： A significant neuroprotective effect was observed in the QKL - ingredient combination treatment group, which was superior to that in the nimodipine treatment group. The expression of BDNF mRNA in the brain was also significantly up - regulated by the QKL - ingredient combination treatment in a rat model of focal cerebral ischemia- reperfusion injury. CONCLUSION： The QKL ingredients show a neuro -protective effect, the mechanism may be related to the up - regulation of BDNF in the brain.