植物雌激素α-玉米赤霉醇对HUVEC低氧/复氧损伤的保护作用

Effects of phytoestrogen α-zearalanol on hypoxia/reoxygenation injury in HUVECs

目的:探讨植物雌激素α-玉米赤霉醇(ZAL)对人脐静脉内皮细胞(HUVECs)低氧/复氧损伤的保护作用及其机制。方法:低氧(93%N2-5%CO2-2%O2)环境中培养HUVECs 3 h后再恢复正常氧供应1 h。用MTT法检测细胞存活率,分光光度法测定细胞培养上清液中LDH、SOD活性及MDA含量。结果:HUVECs经低氧/复氧处理后,细胞存活率及SOD活性显著低于对照(P<0.01),LDH活性及MDA含量显著高于对照(P<0.01);经不同浓度(10-9-10-6mol/L)的ZAL或雌二醇(E2)预处理后,均可显著缓解上述改变,此作用为剂量依赖性,同浓度ZAL与E2的作用无显著差异。结论:ZAL对低氧/复氧损伤的HUVECs可产生与E2类似的保护作用,机制可能与促进自由基清除而减轻细胞氧化应激损伤有关。

AIM： To investigate the effects of phytoestrogen α-zearalanol （ZAL） on hypoxia/reoxygenation （H/R） injury and mechanism involved in human umbilical vein endothelial cells （HUVECs）. METHODS： HUVECs were exposed to hypoxia for 3 hours and then reoxygenation 1 hour. ZAL or 17β - estradiol （ E2 ） at concentrations of 10^-9 - 10^-6 mol/L were pretreated before hypoxia. The survival rate of HUVECs was detected by MTT. Either the activities of LDH and SOD or the level of MDA in supernatant was detected by spectrophotometry. RESULTS： The survival rate of HUVECs and the activity of SOD were significantly decreased （ P 〈 0.01 ） , while the activity of LDH and the level of MDA were significantly increased （ P 〈 0. 01 ） after H/R. These changes were reversed by pretreatment with ZAL or E2 , and there was no significant difference between their effects in the same dose of ZAL and E2. CONCLUSION： These results suggest that phytoestrogen ZAL protects HUVECs from H/R injury by inhibiting the oxidative stress, which was similar to E2.