四逆汤对兔髂动脉球囊损伤病变区TGF-β_1表达的影响

Effects of Sini decoction on the expression of TGF-β_1 in balloon injured iliac artery of rabbits

目的:通过球囊损伤兔髂动脉造成动脉损伤的模型,观察四逆汤对损伤后动脉内膜的保护和修复作用以及对转化生长因子β1(TGF-β1)的影响。方法:雄性新西兰兔24只,随机分为对照组、模型组、四逆汤治疗组(四逆汤组),每组8只。对照组给予普通饲料,模型组和四逆汤组给予高脂饮食。饲养2周后模型组、四逆汤组兔行髂动脉内膜剥脱术。术后4周处死各组实验兔,采用ELISA方法检测血清TGF-β1水平;HE染色观察髂动脉内膜增生情况,通过免疫组化观察TGF-β1蛋白在血管壁的表达;采用RT-PCR方法检测血管壁TGF-β1mRNA的表达。结果:病理观察可见对照组兔髂动脉管腔和管壁厚度正常,管壁光滑;模型组的管壁明显增厚、管腔明显狭窄,内膜增厚;而治疗组管壁增厚较轻,管腔狭窄较轻,内膜增厚程度减轻。对照组兔血清TGF-β1水平明显低于模型组和四逆汤组,四逆汤组又明显低于模型组(P<0.05)。免疫组化发现对照组兔髂动脉内膜TGF-β1的染色灰度和染色阳性面积比明显小于模型组和四逆汤组,而四逆汤组TGF-β1的染色灰度和染色阳性面积比明显小于模型组(P<0.05)。对照组兔髂动脉TGF-β1mRNA表达明显低于四逆汤组和模型组,而四逆汤组TGF-β1mRNA的表达少于模型组(P<0.05)。结论:四逆汤可减轻兔髂动脉剥脱术后的内膜增生和血管的狭窄,其机制可能与抑制髂动脉损伤后内膜TGF-β1的基因和蛋白的表达,减少TGF-β1的产生有关。

AIM： To investigate the effect of Sini decoction （SND） on vascular stenosis and the expression of transfoming growth factor - β1 （TGF - β1 ） in iliac artery balloon injured rabbits. METHODS： 24 male New Zealand albino rabbits were divided into three groups： control group, model group and SND treatment group. The iliac arteries were injured by balloon in model and SND groups. Four weeks later, serum TGF - β1 level was assayed by ELISA. Endothelial hyperplasia, TGF- β1 protein and mRNA expression were observed in injured iliac artery. RESULTS： Light microscope showed that the vascular lumina were narrower, intima was thicker in model group control and SND treatment group. The serum TGF - β1 level was lower in control than model group and SND treatment group, and the serum TGF -β1 level in SND treatment group was lower than that in model group. Immunohistochemistry and RT - PCR results showed that TGF - β1 protein and mRNA expression was lower in rabbit iliac artery of control group than that in model group and SND treatment group, and the expression of TGF- β1 protein and mRNA decreased significantly in SND treatment group compared with model group. CONCLUSION： SND could lessen intimal hyperplasia and vascular stenosis in balloon injured iliac artery, which might be related to decrease in TGF - β1 protein and gene expression in iliac artery.