全反式维甲酸对结肠癌不同增殖潜能细胞株VEGF表达的影响

Effect of all trans retinoic acid on VEGF expression in colorectal carcinoma cell strain with different proliferation potential

目的:探讨全反式维甲酸对结肠癌不同增殖潜能细胞株VEGF表达的作用;研究VEGF在结肠癌侵袭和转移中的作用。方法:采用细胞培养观察、ATRA干预、MTT和FACS方法确定结肠癌细胞株CW-2和LS174T的生长增殖状况,用Northern b lotting方法检测结肠癌中VEGF mRNA的表达量,用免疫细胞化学观察细胞VEGF蛋白的表达。结果:MTT生长曲线显示结肠癌细胞株LS174T的生长增殖比CW-2快;FACS结果显示LS174T细胞的S期细胞较CW-2细胞数多;Northern b lotting和免疫细胞化学检测在CW-2中有明显的VEGF表达,但在高增殖细胞株LS174T中VEGF的表达更明显。结论:VEGF在结肠癌细胞株中有较高的表达。在高增殖结肠癌细胞株VEGF表达更明显。ATRA可能通过抑制VEGF表达,而抑制结肠癌细胞的增生。

AIM： To investigate the Effects of ATRA on vascular endothelial growth factor （VEGF） expression in colorectal carcinoma cell strain with different proliferative potential, and to study the role of VEGF in colorectal carcinoma with invasion and metastasis. METHODS： The state of proliferation in CW-2 and LS174T cell strain were investigated by using cell culture, ATRA intervention, MTT and FACS. Quantitative expression of VEGF mRNA in colorectal carcinoma was detected by Northern blotting. The protein expression of VEGF was observed by immunohistochemistry. RESULTS ： Growth curve of MTT showed that proliferation of LS174T cell strain was faster than those of CW -2, FACS showed that the cells in S phase of LS174T cell strain were more than those of CW - 2. Detection by Northern blotting and immunohistochemistry showed that the expression of VEGF mRNA in LS174T cell strain was obviously higher than those in CW -2. Furthermore, ATRT at a dose of 10-s mol/L significantly inhibited cell proliferation and VEGF expression in LS1747 cell strain, CONCLUSION： VEGF promotes angiogenesis and increases blood supply in colorectal carcinoma, which is closely correlated with tumor growh, all - trans retinoic acid （ ATRA ） may restrain cell proliferation of colorectal carcinoma through down regulation of VEGF expression.