摘要
目的观察地塞米松对小鼠骨髓基质干细胞体外增殖的作用。方法取雄性6周龄ICR小鼠股骨骨髓基质细胞进行体外培养,在细胞培养不同时间点加入10-8mol/L地塞米松(实验组)或保持基础培养条件(对照组),用CellTiter方法分别检测两组的细胞增殖情况,并予比较。结果在细胞培养不同阶段开始应用地塞米松的实验组中,细胞增殖能力较对照组降低,随着培养时间延长实验组细胞数量明显少于对照组。实验组中细胞形态较对照组更趋成熟。结论地塞米松在促进骨髓干细胞定向分化早期抑制骨髓基质干细胞体外增殖。
Objective To study the mechanism of stem cells proliferation using dexamethasone-induced mouse bone marrow culture system. Methods Bone marrow cells were derived from 6-week-old ICR mice. Dexamethasone was initiated in experimental group at different time in the cultures. The control group was treated with placebo. Cell number was counted and compared between the two groups by CellTiter kit. Results The number of cells in the experimental group was significantly less than that in the control group at every time points. Much more cell differentiation was observed in＇the experimental groups. Conclusion Dexamethasone inhibits stromal cell proliferation while stimulates osteoblast differentiation.
出处
《中国骨质疏松杂志》
CAS
CSCD
2006年第5期452-454,共3页
Chinese Journal of Osteoporosis
基金
国家自然科学基金资助项目(30440077)
关键词
骨髓基质干细胞
成骨细胞
地塞米松
细胞增生
细胞分化
Bone marrow stem cell
Osteoblast
Dexamethasone
Cell proliferation
Cell differentiation
