联合补充大豆异黄酮和钙预防去势大鼠骨质疏松的实验研究

Effects of soy isoflavones combined with calcium on osteoporosis in ovariectomized rats

目的研究联合补充大豆异黄酮和钙对去卵巢大鼠骨质疏松的预防作用。方法将50只3月龄SD雌性大鼠摘除卵巢后按体重随机分成5组:假手术对照组、手术对照组、大豆异黄酮组(40mg/kgBW)、钙组(100mg/kgBW)、钙+大豆异黄酮组(Ca100mg/kgBW+SI40mg/kgBW)。连续灌胃饲养3个月后测定大鼠血清骨钙素,左侧股骨测定骨密度和骨钙含量、右侧股骨进行骨组织形态学计量分析。结果Ca+SI组血清BGP水平和股骨骨密度均高于其他去卵巢各组(P<0·05),但低于Sham组(P<0·05);Ca+SI组大鼠股骨的骨钙含量与Ca组、Sham组差异均无统计学意义;在去卵巢各组大鼠中手术对照组、大豆异黄酮组和钙组大鼠的骨小梁数目和骨小梁厚度显著减少,骨小梁间距明显增宽,与钙+大豆异黄酮组相比有显著差异(P<0·05)。结论与单独补充大豆异黄酮或钙相比,大豆异黄酮与钙的联合应用能够更有效地预防去卵巢大鼠骨质疏松的发生。

Objective To study the preventive effects of soy isoflavones combined with calcium on osteoporosis of ovariectomized rats. Methods Fifty SD female rats, 3 months old, were randomized into 5 groups： （1）shamed control group; （2） operated control group; （3） soy isoflavone group （ovariectomized, i.g. SI 40 mg/kg） ; （4） Ca group （ovariectomized, i.g. Ca 100 mg/kg） ; （5） soy isoflavone ＋ Ca group （ovariectomized, i.g. SI 40 mg/kg and Ca 100 mg/kg）. All rats were sacrificed at the 13^th week. Femur bone mineral density（BMD）, bone calcium, serum osteocalcin and biomechanical properties were measured. Results The mean serum osteocalcin level and femur metaphysic BMD of Ca ＋ SI rats were higher than that of the other ovariectomized rats （ P 〈 0.05 ）, but lower than that of the sham rats （ P 〈 0.05） ; bone calcium of Ca ＋ SI rats did not show any difference to that of the Ca group, neither with the sham group （ P 〉 0.05 ）. Compared with Ca ＋ SI rats, the other ovariectomized rats had less bone trabecula （P 〈 0.05 ）. Conclusion Soybean isoflavones in combination with Ca is better in osteoporosis prevention in ovariectomized rats than soybean isoflavone or Calcium supplementation alone.