干扰素α应答基因的克隆及在兔早期胚胎发育中功能分析

Molecular Cloning and Functional Analysis of IFRG in Rabbit Oocytes and Preimplantation Embryos

干扰素(interferon,IFN)在哺乳动物早期胚胎发育过程中具有重要的生理功能,但是其作用机制尚不清楚.通过筛选卵巢cDNA文库和5′-RACE方法,克隆了兔卵巢干扰素α应答基因(interferonresponsivegene,IFRG)的全长cDNA(登录号:AJ584672).利用RT-PCR证明IFRG在兔卵母细胞和植入前胚胎中均有表达,这将为深入研究IFN在早期胚胎中的作用机制提供理论参考,卵巢原位杂交表明IFRG在成熟卵泡(类型5)的颗粒细胞中有表达,在初级和次级卵泡的膜鞘细胞和粒层细胞中有很高的表达,鉴于这些细胞与卵泡的发育密切相关,推测IFRG在卵泡的发育、成熟和排卵中发挥重要作用.

Interferons （IFNs） play important physiological roles in early embryo development, but its mechanism is still unclear. The full length cDNA of interferon responsive gene （IFRG） was cloned in rabbit ovary （Accession No. AJ584672）. The expression profile of IFRG in oocytes and preimplantation embryos was detected using RT-PCR. IFRG was expressed in oocytes and preimplantation embryos in rabbit, which provided some clues for better understanding the role of IFNs during embryo development. In situ hybridization showed that IFRG was highly expressed in granulosa, thecal and cumulus cells, which were consanguineous interrelation to follicles development. So it was proved that IFRG expression was involved in the follicles development, maturation and ovulation in rabbit.