一氧化氮不介导内毒素引起的离体大鼠脊髓释放降钙素基因相关肽

NITRIC OXIDE IS NOT IN ENDOTOXIN-INDUCED RELEASE OF CALCITONIN GENE-RELATDE(CGRP)FROM RAT SPINAL CORD IN VITRO

目的：探讨一氧化氮（ＮＯ）及ＮＯ合成酶（ＮＯＳ）抑制剂对内毒素引起的大鼠脊髓降钙素基因相关肽（ＣＧＲＰ）释放的影响。方法：采用离体大鼠脊髓切片孵育，以放射免疫法测定ＣＧＲＰ的释放。结果：ＮＯＳ的合成底物Ｌ－Ａｒｇ；ＮＯ供体ＳＮＡＰ和ＳＮＰ；各种ＮＯＳ抑制剂Ｌ－ＮＮＡ，Ｌ－ＮＡＭＥ，Ｌ－ＮＭＭＡ和７－ＮＩ对离体大鼠脊髓ＣＧＲＰ的基础释放以及内毒素引起的ＣＧＲＰ释放均无明显影响。结论：ＮＯ在离体脊髓水平没有参与内毒素引起的ＣＧＲＰ释放过程。

Objective: The effects of nitr ic oxide(NO)and NO synthase inhibitor on endotoxin(ETX)-in- duced CGRP release from rat spinal cord in vitro were studied.Methods:Using spinal cord slices in rats and radioimmunoassay of CGRP。Results:Substrata of NO synthase:L-arginine;NO donor:sodium nitroprusside(SNP),S-nitroso-N-acetylpenicillamine(SNAP);NO synthase inhibitor:NG-Nitro-L- arginine(L-NNA),NG-nitro-L-arginine methyl ester (L-NAME),NG-monomethyl-I-arginine acetate(L-NMMA),7-Nitroindazole had no effects on basel and the ETX-induced CGRP release from spinal cord in uitro.Conelusion:The data suggest that NO is not involved in the ETX-induced CGRP release from rat spinal cord in uitro;