谷氨酸钠致使新生大鼠海马锥体细胞损伤的形态学研究

Morphological Research on the Damage of the Hippocampal Pyramidal Cell in the Neonatal Rat Following Injection with Monosodium Glutamate

追踪观察谷氨酸钠（ｍｏｎｏｓｏｄｉｕｍｇｌｕｔａｍａｔｅ，ＭＳＧ）处理后的新生大鼠不同生长期海马锥体细胞的形态学变化。发现海马锥体细胞的损伤主要有急性期细胞肿胀和迟发性细胞固缩２种类型。急性期细胞损伤主要发生在１１日龄动物，细胞核急骤肿胀，细胞溃变死亡。以ＣＡ_１区最为明显，ＣＡ_４区除细胞肿胀外，尚伴有部分细胞固缩。齿状回细胞在此时期无明显变化。５２日龄组和１００日龄组动物主要表现为ＣＡ_２～ＣＡ_４区细胞核缩小，染色质聚集成块，胞浆浓缩深染，细胞固缩死亡。齿状回近门处内层细胞固缩，小颗粒细胞活性增加，数目明显增多。ＣＡ_１区较少发现细胞固缩现象，但背侧细胞减少明显。

It was the study on the tracing observation in different living periods after the hippocampal cells in the neonatal rat was damaged after subcutaneous injection with monosodium glutamate (MSG). Injuries were classified into two types. The results were as follows: ①The cell swelling in acute period. Injuries of cell mainly presented in the rats of 11 days. The nuclei was swellen,and the cell degenerated even into death. There were swelling in varying degrees in the pyramidal cells of CA1～CA4 region ,especially in that of CA1 region. In CA4 region, as well as swelling, some cells appeared pyknostic. Dentatus gyrus neurons had no significant change in this period. ②Delayed cell pyknosis. Nuclei contraction,chromoplasm congulation,cytoplasm contraction and aridity dark for the stain,cell pyknosis and death were observed in CA2～CA4 region of rats of 52 days and 100 days. The activity and number of pyknosis granule cells in the inner layers increased apparently near the door of dentatus gyrus. Although the phenomenon of pyknosis could be observed scarcely in CA1 region, the number of dorsal cells decreased significantly.