高效液相色谱法测定钻山风颗粒中肉桂酸的含量

Determination of cinnamicaid in the Zuanshanfeng granules

目的：建立钻山风颗粒中肉桂酸的含量测定方法。方法：采用高效液相色谱法，色谱柱：大连依利特Hypersil BDS C18柱（4．6mm×200mm，5μm）；流动相：乙腈-0．1％磷酸溶液（25：75，v／v）；检测波长：278nm；柱温：25℃；流速：1mL·min^-1结果：在0．11464～1．83424μg范围内进样量与峰面积积分值有良好线性关系．回归方程为Y=5×10^6X＋97402．r=0．9999（n=6）。结论：方法简便可行，准确可靠，可用于钻山风颗粒中肉桂酸的含量测定。

OBJECtiVE To establish a method for the determination of cinnarnicaid in the Zuanshanfeng granules. METHODS The HPlC determination was perforated with the column of Dalian Hypersil BDS C18 （4. 6 mm× 200 mm,5μm） and mobile phase of acetonitrile -0. 1 % phosphate dibasic anhydrous （25：75, v/v） at a flow rate of 1 ml,/min, and the detection was conducted at 278 nm, the temperature of the column was 25 ℃. RESULTS The method showed a good linear relatiorkship in the range of 0. 114 64- 1. 834 24μg. The reeursive equation was..Y= 5 × 10^6X＋ 97 402,r=0. 9999 （n= 6）. CONCLUSION This method is praeticable,eonvenient,preeise and reliable. It can be applied to the determination of einnamicaid in the Zuanshanfeng granules.