高效液相色谱法测定健康人体血浆中罗红霉素的浓度

Determination of roxithromycin in human plasma by HPLC

目的：建立人血浆中罗红霉素浓度的HPLC测定方法。方法：采用反相高效液相色谱法检测人血浆中罗红霉素的浓度：Kromasil C18色谱柱（瑞典，4．6mm×150mm，5μm），流动相：30mmol·L^-1醋酸铵（含1％的三乙胺．磷酸调pH值至7．8）：乙腈。49：51，流速为1．0mL·min^-1．进样量40μL．柱温40℃，检测波长220nm。结果：标准曲线线性范围为0．25～32．0mg·ml^-1（r=0．9990,n=8）。血浆中罗红霉素的定量下限为0．25mg·mL^-1．日内RSD为5．05～6.47%，日间RSD为4．53～7．82％，萃取回收率为64．58～83．30％。结论：该方法简单，快速，重现性好．适用于人血浆中罗红霉素浓度的检测。

OBJECTIVE To establish a method for determining roxithromycin in human plasma by high performance liquid chromatography. METHODS Roxithromycin was successfully determined by reversed-phase high-performance liquid chromatography. Kromasil C18 column（150 mm×4. 6 mm,5 μm） was used. The mobile phase consisted of 30 mmol·L^-1 ammonium acetate solution containing（ 1 % triethylamine, phosphoric acid, pH = 7. 8）-acetonitrile （49：51）. All separation were at 40℃. and the flow rate was 1. 0 mL·min^-1 , Injection volume was 40 ILl.. The peak of roxithromycin was monitored to set at UV 220nm. RESULTS The linear range of roxithromycin was 0. 25-32. 0（g · mL^-1 （r= 0. 9990,n = 8）. The detection limit was 0. 25 mg· mL^-1. The RSD of intra-day tests were 5.05-6. 47% and that of inter-day tests were 4. 53-7. 82%. The recovery of roxithromycin was 64. 58-83. 3%. CONCLUSION The method is simple, rapid and producible. It is suitable for determination of roxithromycin in human plasma.