饲养细胞促进人胚脑神经细胞体外扩增的动物实验

The study on feeded cells promoting human embryonic neurocytes proliferation in vitro

目的探讨饲养细胞对人胚脑神经细胞体外培养的作用。方法取妊娠12～16周合法人工流产胎儿大脑皮层,用DMEM培养液制备神经细胞悬液。接种于预先制备的饲养细胞、铺有聚L-赖氨酸和没有经过上述处理的培养板上进行原代人胚脑神经细胞培养。倒置显微镜下观察神经细胞的生长发育、增殖分化、细胞迁移、形态学变化等,免疫组织化学Streptavidin peroxidase(SP)法鉴定神经细胞。结果饲养细胞组神经细胞贴壁时间早于对照组(P<0．05),神经细胞迁移速度、胞体大小、突起长度均高于对照组(P<0．05)。结论饲养细胞具有促进人胚脑神经细胞贴壁、生长以及迁移的作用。

Objective To study the effect of rat feeded cells on human embryonic neuroeytes cultured in vitro.Methods The primary culture of the human embryonic cortex neurocytes of 12-16 weeks was carried out in vitro by feeder cells,L-polylysine and blank control respectively.The growth,differentiation and transference of neurocytes were observed by using microscopy and identified neurocytes and by Streptavidin peroxidase （SP）. Results In the feeded cells group,the time of neurocytes sticking to wall and neuron -specific enolase（NSE） was earlier （P〈0.05）.The moving speed,the area and the perimeter of the cells were bigger and higher（ P〈0.05 ）.Conclusion The rat feeded cells could promote the human embryonic neurocytes growth , stiekinff tO wall and moving.