环核苷酸及［Ca^（2＋）］i介导β－内啡肽刺激大鼠巨噬细胞IL－1的分泌

Activation of IL-1 Secretion by β-EP via Cyclic Nucleotides and [Ca￣(2+)]i in Rat Macrophages

本实验初步观察β－ＥＰ刺激ＩＬ－１分泌的第二信使，培养的大鼠腹腔巨噬细胞，分别加入β－ＥＰ（１）组、β－ＥＰ＋ＮＬＸ（２）组、β－Ｅｐ＋ｆｏｒｓｋｏｌｉｎ（３）组、β－ＥＰ＋亚甲兰（１）组、β－ＥＰ＋尼凡地平（５）组、β－ＥＰ＋ｆｏｒｓｋｏｌｉｎ＋亚甲兰＋尼凡地平（６）组、主理盐水（７）组。２４ｈ后收集巨噬细胞及上清液检测有关指标，发现：第１组细胞内ｃＡＭＰ显著降低，ｃＧＭＰ、［Ｃａ￣（２＋）］ｉ明显升高，上清液呈强烈的ＩＬ－１活性；第２、６、７组细胞内ｃＡＭＰ、ｃＧＭＰ、［Ｃａ￣（２＋）］无明显变化，上清液无ＩＬ－１活性；第３、４、５组上清液们有较强的ＩＬ－１活性，而细胞内ｃＡＭＰ、ｃＧＭＰ及［Ｃａ￣（２＋）］ｉ呈不同变化，提示：ｃＡＭＰ，ｃＧＭＰ及［Ｃａ￣（２＋）］ｉ可能是β－ＥＰ刺激巨噬细胞分泌ＩＬ－１的第二信使。

he peritioneal macrophages from SD rat were respectively challenged in culture with β-EP(1),β-EP+NLX(2),β-EP+forskolin(3),β-EP+methylene blue(4),β-EP+nifandipine(5),β-EP+forskolin+methylene blue+nifandine(6)and saline(7)separately,24 hours later,the levels of cAMP,cGMP and[Ca￣(2+)]i in macrophage andsupernate were detected.The results suggested that there were a remarkable lowered cAMP and elevated cGMPand[Ca￣(2+)]i in cells.and a strong IL-1 activity in supernate in group 1.In group 3,4,5,a significant activity alsoexisted in IL-1 of supernate,but a different changexin cAMP,cGMP,[Ca￣(2+)]i of cell.While no obvious changeswere observed in all parameters as abOve in group2,6,7.It s suggested that cAMP,cGMP,as well as[Ca￣(2+)]may all be the second messengers through which β-EP stimulated IL-1 spcretion.