催化动力学荧光猝灭法测定痕量铜(Ⅱ)研究

Study on determination of trace copper(Ⅱ) by catalytic kinetic fluorimetric quenching method

研究了在NH3·H2O-NH4Cl缓冲介质中，痕量Cu（Ⅱ）对过氧化氢氧化荧光剂吖啶橙而荧光猝灭反应存在明显的催化作用，由此建立了动力学荧光法测定痕量铜的新方法。方法的线性范围为0．008-0．16μg／L，线性回归方程△F=9．1＋170．9c（μg／25mL），相关系数r=0．9992。检出限为0．0016pg／L。用本方法测定自来水和茶叶中的铜结果满意。

A kinetic-fluorimetric quenching method for detennination of trace Cu （Ⅱ ） was developed in this paper. The method was based on the catalytic effect of Cu（ Ⅱ ） on oxidation of acridine orange by hydrogen peroxide in a NH3. H2O-NH4C1 buffer medium. The linear range of determination for Cu （Ⅱ） was 0.008 - 0.16 μg. L- l with a detection limit of 0.001 6 μg. L- 1 and AF = 9. 1 ＋ 170.9 c（μg/25 mL）, r = 0.999 2. The method could be applied to determine trace copper in tea and tap water samples with satisfactory results.