用原位聚合酶链反应方法检测结节病病理组织中博氏疏螺旋体的研究

DetectionofborreliaburgdoferiDNAgranulomatoustissuesfrompqtientswithsarcoidosisusingpoly┐merasechainreactioninsituteachnique

目的进一步探讨结节病与博氏疏螺旋体（Ｂｂ）感染之间的关系。方法用原位聚合酶链反应（ＰＣＲ）和免疫荧光方法分别对２３例结节病患者病理活组织检查（活检）组织中Ｂｂ鞭毛ＤＮＡ和５５例血清标本中Ｂｂ抗体进行了检测。结果（１）２３例病理组织中未有１例检测到Ｂｂ鞭毛ＤＮＡ的存在。（２）５５例结节病患者血清中Ｂｂ抗体总阳性率为５５％，明显高于正常人的１０％（Ｐ＜０．００５）。结论Ｂｂ可能不是结节病的病因，结节病患者血清Ｂｂ抗体的升高。

ObjectiveToinvestigatethecorrelationbetweensarcoidosisandBoreliaburgdorferi(Bb)infection.MethodsFlagelaDNAofBbweredetectedin23tissuespecimensfrompatientswithconfirmedsarcoidosisusingpolymerasechainreactioninsitutechnique(insituPCR)andtheantibodiestoBbwereexaminedin55serumsamplesobtainedfromthepatientsbyindirectimmunoflurescenceas-says.Results(1)NoneofgranulomatoustissueswasfoundtohaveBbDNAin23tissuesamples.(2)30of55(54.6%)patientswithsarcoidosiswerefoundantibodiestoBbpositive,incontrast,sixof60(10%)normalsubject-shadantibodiesagainstBb,thepositiveratewasremarkablyhigherinpatientgroupthanthatinhealthygroup(P<0.005).ConclusionsBbmightnotbethecausativeagentofsarcoidosis,theelevatedtitresofserumantibodiesagainstBbinpatientswithsarcoidosisisanonspecificresponse.