聚合酶链反应试剂对检测痰结核分支杆菌的影响

EffectsofreagentsusedinpolymerasechainreactiononsensitivityansspecificityofdetectionofMy┐cobacteriumtuberculosis

目的观察聚合酶链反应（ＰＣＲ）的试剂对检测痰结核分支杆菌的敏感性和特异性的影响。方法对结核病组２０５例和非结核病组１０５例的痰标本同时进行三个厂家生产的ＰＣＲ试剂检测、普通细菌培养和抗酸染色法涂片检测。再对ＰＣＲ假阳性的痰普通细菌培养结果进行分析，试找出ＰＣＲ假阳性的其它因素。结果三种不同引物序列和扩增产物片段长度的ＰＣＲ试剂对结核性痰标本的阳性率分别为８２．４％、７１．７％和６１．０％，组间差异有非常显著意义（Ｐ＜０．００５）。

ObjectiveTOexploretheefectsofreagentsusedinpolymerasechainreactiononsensitivityandspecificityofdetectionofmycobacteriumtuberculosis.Methods205tuberculosisand105nontuberculosiscases'sputumspecimenswereexaminedsimultaneouslybysmear,polymerasechainreactionusingthreediferentreagentsandculture.ResultsThepositiveratesofMycobacteriumtuber-culosisbypolymerasechainreactionusingthreediferentreagentswere82.0%,71.7%and61.0%re-spectivelyin205tuberculosiscases,while18.0%,11.4%and2.8%respectivelyin105nontuberculosiscases.Thediferencebetweentheabovethreediferentgroupswasstatisticalysignificant(P<0.005).ConclusionsThesequencesofprimersandthelengthofamplifiedproductsofdiferentreagentsmaybeassociatedwiththepositiveandthefalsepositiveratesofMycobacteriumtuberculosisinpolymerasechainreaction.