阳离子多聚体信号放大方法应用于压电基因生物传感器检测铜绿假单胞菌的研究

Piezoelectric Gene Biosensors for Detection of Pseudomonas aeruginosa Using Signal Amplification Method with Cationic Conjugated Polymers

目的探讨压电基因生物传感器引入阳离子多聚体(脂质体)信号放大方法,快速检测铜绿假单胞菌的可行性,建立一种新的利用静电吸附的压电信号放大方法。方法在基因压电石英传感器阵列固定针对铜绿假单胞菌的寡核苷酸探针,加入靶DNA与之杂交反应,检测频率变化值,然后在杂交后的检测体系中加入不同浓度的脂质体,检测频率变化值,并与对照组进行比较。结果加入阳离子多聚体后,频率变化值明显增大,与对照组相比较差异有统计学意义(P<0.05),并且阳离子多聚体最适使用浓度为0.8μg/μl。结论阳离子多聚体可有效地放大压电传感信号。

OBJECTIVE To discuss the feasibility of signal amplification method with cationic conjugated polymers （liposome） applied during the detection of Pseudomonas aeruglnosa using piezoelectric gene biosensors, METHODS Oligonucleotide probe for P, aeruginosa was immobilized on the surface of gene sensor array and hybridized by PCR production of P, aeruginosa, After hybridization, liposome was added, The frequency shifts were recorded and compared with those ones of the control groups, RESULTS The frequency shifts were significantly increasing when adding liposome and the compatible concentration of liposome was 0.8μ/μl. CONCLUSIONS Liposome signal amplification is proved to be an effective method to amplify the piezoelectric signal.