漏声表面波生物传感器液相检测系统的构建及检测HPV的实验研究

Design of a Detection System of Leaky Surface Acoustic Wave Biosensor in Liquid Phase and Test of HPV

目的建立漏声表面波生物传感器液相检测技术平台,并用该检测系统实时检测人乳头瘤病毒(HPV)。方法构建检测和参比双路延迟线,并观察在液相中两路延迟线的相位变化规律;以人乳头瘤病毒为检测对象,用传感器检测系统对其进行实时检测。结果两路延迟线的相位值在液相中发生不同的变化;检测HPV时,检测延迟线的相位值发生较大的变化,而参比通道的相位曲线变化不是很大。结论成功构建了漏声表面波生物传感器液相检测技术平台,并实现对双链DNA病毒HPV实时检测反应。

OBJECTIVE To design the detection system of the leaky surface acoustic wave（LSAW） biosensor in liquid phase and detect human papilloma virus（HPV） target sequence with the LSAW biosensor. METHODS A detection delay line and a reference delay line were constructed, the phase of the two delay line changes was evaluated in liquid phase, and real time monitoring of HPV reaction was performed by using the LSAW biosensor system. RESULTS The phase of the two delay lines had different changes in liquid phase; HPV could lead to a great change of the phase in the detection delay line, and reference delay line had no change. CONCLUSIONS The detection system of the LSAW biosensor in liquid phase is successfully constructed and real time monitoring of detection of HPV is realized.