摘要
应用C30-HPLC-PDA,万寿菊花萃取物中的叶黄素类化合物获得了良好的分离,包括:叶黄素酯的分离和单体几何异构体的分离。色谱条件为:WatersYMCCarotenoidS-5(4.6×250mm)柱;乙腈-甲醇(75:25,V/V)为流动相A,甲基叔丁基醚为流动相B,线性梯度洗脱;流速:1.0ml/min;PDA波长范围:300～600nm;进样量:20μl。根据各组分的色谱行为、光谱特征和在碘催化下发生几何异构的产物分析对各组分进行初步鉴定。实验结果显示:C30固定相在分离万寿菊花萃取物中的叶黄素类化合物的应用中有良好的前景。
The lutein and related compounds from the flower of marigold were readily separated by C3o-HPLC-PDA, including the separation of lutein esters and geometrical isomers, under following conditions： With YMCTM Carotenoid S-5 column （250 × 4.6mm）, mobile phase A and B were acetonitrile-methanol （75：25, VN） and MTBE, respectively. Linear gradient elution was performed at the flow rate of 1.0 ml/min. The PDA acquired the spectra of fractions from 300 to 600nm. Sample injection volume was 20 μ 1. According to its chromatographic behavior, spectral characters and geometrical transition catalyzed by iodine, each fraction was preliminarily identified. Result from this study suggested that the application of C30-HPLC-PDA in the separation of lutein and related compounds from marigold flower exhibited a bright future.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2006年第11期424-428,共5页
Food Science
基金
北京市教育委员会科技发展计划面上项目(200411417003)
