气相色谱-质谱(GC-MS)法测定食品中丙烯酰胺研究

Determination of Acrylamide in Food by Chromatography-Mass Spectrometry

研究了食品中丙烯酰胺的气相色谱-质谱(GC-MS)定量测定方法。样品中丙烯酰胺经水、醇类等极性溶剂提取,高速冷冻离心过滤和固相萃取柱净化,溴化衍生后生成2,3-二溴丙烯酰胺(2,3-DBPA),气相色谱-质谱(GC-MS)特征离子定性,主要离子碎片(m/z):152、150、108、106,标准加入法定量。实验结果表明:方法检出限为5～10μg/kg,回收率在80.0%～110.0%,变异系数为3.25%～9.40%,线性范围在0～3.5mg/kg。该分析方法具有准确度高、选择性好、检出限低、线性范围宽、干扰少等特点,十分适合食品中丙烯酰胺定量测定。

An analytical method of cancedgenic acrylamide in food by CJC-MS was studied in this article. The acrylamide in samples was extracted with polar solvents such as water, alcohols and cleaned up by centrifugal titration, solid-phase-extractioncolumn. 2,3-DBPA was derived from the extracts through bromation, and determined by CJC-MS.The major characteristic ion fragments （m/z） are： 152, 150, 108, 106. The limit of detection is 5 - 10 μ g/kg. The recoveriy is 80.0%-110.0%. The variable coefficient is 3.25%-.40%. The linear range is 0-.5mg/kg. This method is precise and selective, low limit of detection, wide liner range, little interference. It is suitable for the determination of acrylamide in food.