摘要
以壳聚糖和海藻酸钠为复合缓释壁材,对葡萄糖氧化酶(GOD)进行微胶囊包被缓释处理,研究了不同因素对缓释葡萄糖氧化酶酶活回收率和缓释作用的影响。结果显示,制备缓释葡萄糖氧化酶的最佳条件为:海藻酸钠浓度2.0%,壳聚糖浓度0.75%,葡萄糖氧化酶浓度0.3%,pH值5.0,钙离子浓度0.3mol/L,搅拌速度1200r/min。此时葡萄糖氧化酶酶活回收率最高,达到93.8%,且显示出较好的缓释性能。
Chitosan/sodium alginat was selected as a compound wall material of controlled- release microcapsule of glucose oxidase in this study. Influence of different factors to the recovery of enzyme activity and the controUed-release process were investigated. The results showed that the optimal conditions to prepare the controlled- release glucose oxidase were concentration of sodium alginate 2.0%, concentration of chitosan 0.75%, concentration of glucose oxidase 0.6%, pH 5.0, concentration of calcium ion 0.3%, stirring speed 1200r/min. Under this condition the recovery of enzyme activity reached 93.8%, and the controUed-release GOD showed better slow release character.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2006年第11期248-253,共6页
Food Science
基金
湖北省自然科学基金项目(2005ABA090)
湖北省教育厅重点项目(D200518007)
关键词
葡萄糖氧化酶
壳聚糖/海藻酸钠
缓释
制备条件
glucose oxidase
chitosan/sodium alginate
controlled-release
preparation condition
