薄层扫描法同时测定南、北五味子提取物中四种木脂素的含量

Simultaneous Determination of the Four Lignans in Extract of Schisandra chinensis and S.sphenanthera by TLCS

目的:建立同时测定南、北五味子提取物中四种主要木脂素含量的方法。方法:分别采用乙醇、超临界CO2提取南、北五味子中的木脂素,采用GF254板,展开剂为正己烷-醋酸乙酯(8:3),双波长薄层扫描(λS=220nm,λR=340nm)测定南、北五味子提取物中五味子醇甲、酯甲、甲素和乙素的含量。结果:五味子醇甲点样量于0.44～1.54μg,酯甲点样量于0.60～2.10μg,甲素点样量于0.52～1.82μg,乙素点样量于0.48～1.68μg范围内与峰面积呈良好线性关系,相关系数R分别为0.9986、0.9960、0.9997和0.9962,平均回收率分别为104.5%、95.0%、96.1%和102.1%,5次测定的RSD分别为4.0%、2.8%、2.6%和1.9%。超萃提取物中四种木脂素含量均高于醇提物。结论:本法简便、快速、精密度较好,可用于同时测定五味子提取物中四种主要木脂素含量。

Objective： To estabfish a method for determining the content of the four lignans in extract of Schisandra chinensis and S.sphenanthera simultaneously. Methods： Samples were extracted with alcohol and CO2-SFE. The contents of schisandrin, Wuweizi ester A, schisandrin A and schisandrin B could be determined simultaneously by dual wavelength TLC- Scanning （λs=220nm, λR=340nm） after the extracts were developed by n-hexane- ethyl acetate （8：3） on self-made GF254 plates. Results： The calibration curves were linear in range of 0.44- 1.54μg for schisandrin（R=0.9986）, 0.60-2.10μg for Wuweizi ester A（R=0.9960）, 0.52- 1.82μ g for schisandrin A（R=0.9997） and 0.48 - 1.68 μg for schisandrin B（R=0.9962） respectively. The average recoveries（n=5） were 104.5% for schisandrin（RSD=4.0%）, 95.0% for wuweizi ester A（RSD=2.8%）, 96.1% for schisandrin A （RSD=2.6%） and 102.1% for schisandrin B（RSD=1.9%）. All of the contents of four fignans in extracts of schisandra with SCF- CO2 are higher than that in extracts of schisandra with alcohol. Conclusion： This method was found to be simple, fast and accurate for simultaneously determining the content of the four lignans in extract of Schisandra chinensisl and S. sphenanthera.