实验性癫痫大鼠血清MBP，脑髓鞘MBP免疫组化和电镜研究

The Serum MBP, Myelin Sheath Immunohistochemistry and Electromicroscope of Expetrimental Epilepsy Rats

为探索脑髓鞘及其髓鞘碱性蛋白（ＭＢＰ）与癫痫发病机理的相关性，作者采用简易的ＭＢＰＥＬＩＳＡ检测实验性癫痫大鼠血清ＭＢＰ含量，同时进行脑ＭＢＰ免疫组化和髓鞘电镜观察。结果显示：癫痫大鼠血清ＭＢＰ含量高于阈下刺激组和正常组（Ｐ＜０．０５），与临床癫痫患者血清ＭＢＰ增高的结果类似；癫痫大鼠脑海马区ＭＢＰ免疫组化和髓鞘电镜观察结果均显示部分区域原有髓鞘规则的多层膜结构松解、紊乱。由此表明癫痫不仅伴有髓鞘损害和脂质组成变化，同时还伴有蛋白质如ＭＢＰ组成的改变和释出，从而影响神经冲动精确定向和快速传导。

We detected the level of myelin basic protein (MBP) in the serum of experimental epilepsy rats using simplified MBP ELISA, and the myelin sheaths of their brain with MBP immuno histochemistry (IHC) and transmitted electronmicroscopy(TEM) in order to explore the relation of brain myelin sheaths and MBP to the pathogenic mechanism of epileptic seizure. The results show that the serum MBP level in the experimental epilepsy group is significantly higher than that in the control groups ( P <0.05), and is similar to that in some patients with generalized myoclonic seizures reported by us previously. The IHC and TEM findings in the brain of epilepsy rats indicate that the lamelliform structure of myelin sheaths in some part of hippocampus region becomes relaxing, disorderly and homogenized. Our studies imply that epileptic seizures are associated with MBP release from the subtle damaged regions of myelin sheaths during epileptic seizures, and the change of MBP component in myelin sheath may play and important role in the pathogenesis of epilepsy.