中线恶网的T细胞受体基因重排分析——14例PCR研究

T Cell Receptor Gene Rearrangement Analysis of Midline Malignant Reticulosis∶ A PCR Study of 14 Cases

应用ＰＣＲ方法对１４例中线恶网进行Ｔ细胞受体β链（ＴＣＲ－β）基因重排分析的结果显示，１２例（８５．７％）有单克隆型的ＴＣＲ－β基因重排；１０例淋巴结Ｔ细胞性淋巴瘤仅有６例有ＴＣＲ－β基因重排。反应性淋巴增生性则为多克隆型重排。该实验从基因水平证实了中线恶网为来自Ｔ细胞的单克隆性肿瘤性疾病。用ＴＣＲ－β通用引物行ＤＮＡ扩增是检测Ｔ细胞性的克隆性增生的方便、快速的方法。

Using PCR method, we analyzed the gene rearrangement of T cell receptor chain (TCR β) in 14 cases of midline malignant reticulosis (MMR). Our results showed that there were non germline, monoclonal rearrangements of TCR β in 12 of 14 cases of MMR(85.7%). In contrast, the same rearrangement pattern was found only in 6 of 10 cases of lymph node based T cell lymphoma. The cases of reactive lymphoid hyperplasia were of a polyclonal rearrangement pattern. This study suggests that MMR is a monoclonal T cell neoplasm and PCR method is a rapid, facilitated and useful method for the detection of clonalities of T cell proliferations.