骨桥蛋白在食管鳞癌中的表达及其临床意义

Expression and clinical significance of osteopontin in esophageal squamous cell carcinoma

目的观察骨桥蛋白(OPN)在食管鳞癌组织、癌旁组织和转移淋巴结的表达状况,探讨OPN在食管鳞癌中表达的临床意义。方法运用免疫组化S-P法检测44例食管鳞癌组织、癌旁组织和20例转移淋巴结免疫组化染色。结果OPN在食管癌组织、癌旁组织及转移淋巴结中的表达率分别为86.3%、0%和100%。癌组织中OPN主要表达于肿瘤细胞的细胞浆中。OPN的表达与肿瘤TNM分期、淋巴结转移状态有关,而与肿瘤位置、肿瘤直径、浸润深度及病理学分级无关。OPN在癌组织、癌旁组织及转移淋巴结表达强度亦存在显著性差异。结论食管鳞癌组织中OPN主要由肿瘤细胞产生。OPN与肿瘤的浸润、转移有关,反映了肿瘤的生物学特性。

Objective To observe the expression of osteopontin （OPN） in tumor tissue, tumor adjacent tissue, and metastasized lymph nodes of esophageal squamous cell carcinoma, and to explore the relation between OPN and clinicopathological characteristics. Methods S-P staining was used to investigate the expression of OPN in 44 cases of the tumor tissue, 44 cases of the tumor adjacent tissue, and 20 cases of the metastasized lymph nodes of esophageal squamous cell carcinoma. Results The positive rate of OPN expression in tumor tissue, tumor adjacent tissue, and metastasized lymph nodes were 86.3%, 0%, and 100%, respectively. OPN was mainly distributed in cytoplasm of tumor cells and related with clinical stage and lymph node metastasis status, but not with tumor location, diameter, infiltration, or pathological grading. There was significant difference of OPN expression among tumor tissue, tumor adjacent tissue, and metastasized lymph nodes. Conclusion OPN is mainly produced by tumor cells and closely correlated to tumor progression.