癌细胞和人血管内皮粘附与器官特异转移相关

The Correlation between Adhesion of Human Cancer Cells and Microvesscular Endothelium and Organ-specific Metastasis

目的:检测5种不同类型的人肿瘤细胞系与4种不同来源的人微血管内皮的粘附,并探讨粘附作用与肿瘤器官特异性转移的相关性。方法:人微血管内皮培养形成完全汇合的静止内皮单层后,与CalceinAM标记人肿瘤细胞系进行粘附测定。结果:8种食管癌细胞系中,有6种与人肺内皮的粘附显著强于与肝窦内皮的粘附;7种食管癌细胞系与人正常食管及食管癌内皮的粘附均强于与人正常肺内皮和肝窦内皮的粘附,这与食管癌临床上肺转移多于肝转移,但相对于远处转移,更倾向于食管内侵袭播散性转移的特征一致。4种结肠癌细胞系与人肝窦内皮粘附均显著强于与人肺内皮和食管正常内皮,与临床上结肠癌主要发生肝转移的特征一致。肝癌、胃癌、肺癌细胞系与内皮的粘附也与临床上转移特征一致。结论:不同组织类型肿瘤细胞与器官来源不同的人内皮细胞的粘附能力具有明显的组织特异性和选择性,且这种粘附特性与临床上肿瘤的组织特异性转移密切相关。阻断这种粘附作用有可能治疗肿瘤转移。

Objective： To determine the adhesion between 5 different types of human tumor cell lines and 4 microvascular endothelia of various sourcesand to analyze the possible correlation between the adhesive capacity and organ-specific metastasis. Methods： After formation of the completely confluent and quiescent endothelial monolayer through microvascualr endothelial culture, the adhesion determination was conducted as the tumor cells were labeled with Calcein AM. The tumor cells attached to endothelium were quantified after incubation and rinsing. Results： Of the 8 esophageal carcinoma cell lines, adhesion between 6 cell lines and human pulmonary endothelium was significantly higher than that of the endothelium of sinus hepaticus, and adhesion between 7 cell lines and normal human esophagus as well as endothelium of the esophageal carcinoma was higher than that of the endothelium of normal human lung and of sinus hepaticus. This was in accordance with clinical characteristics of the esophagus, i.e., pulmonary metastasis was more than liver metastasis and the invasive and disseminated metastasis in the esophagus was much more common compared to distant metastasis, etc. The adhesion between 4 colon carcinoma cell lines and human sinus hepaticus endothelium was significantly higher than that of the endothelium of human lung and of the normal esophagus, which was with according to clinical characteristics of colon carcinoma with liver metastasis. The adhesion between the cell lines of the carcinomas, such as liver cancer, gastric carcinoma and lung cancer, and endothelium was in accordance with the characters of clinical metastases. Conclusion： The adhesive capacity of the human endothelial cells with the tumor cells of different types and organs of various sources has a clear tissue specificity and selectivity and the adhesiveness is closely in relation with clinical tissue specificity of the tumor. To block up the adhesive attraction may be helpful in treating the tumor metastasis.