线粒体DNA转录表达与胃癌发生关系的研究

The Studies on Relationship between Expression of Mitochondrial DNA Transcription and Gastric Carcinogenesis

目的:检测胃癌组织线粒体DNA的转录表达水平变化,探讨mtDNA转录表达变化与胃癌发生的关系。方法:应用RT-PCR法检测42例配对的胃癌和癌旁正常胃粘膜组织的线粒体编码基因COⅠ、ND4、ND5、cyt-b和ATP-6的转录表达差异,并以β-actin作为定量标准物,最后行琼脂糖凝胶电泳。结果:胃癌组织线粒体COⅠ和ND4的转录水平显著高于远癌正常胃粘膜组织,P<0.01;胃癌COⅠ和ND4的表达水平与胃癌的组织分化程度呈负相关;肠型胃癌ND4和COⅠ的表达高于弥漫型胃癌,P<0.05。结论:胃癌的发生需要线粒体提供的某些转录物和蛋白以维持其恶性表型所需要的能量和(或)其他物质,而且分化程度愈低愈需要这种活动以增强其恶性生物学表型和行为。

Objective： To detect the transcriptional expression of mitochondrial genome （mtDNA） in gastric cancer and matched normal gastric mucosa and to further discuss the role of NA transcripts in gastric carcinogenesis. Methods： Reversed-transcription PCR （RT-PCR） was applied to detect the transcripts of CO Ⅰ , ND4, ND5, cyt-b and ATPase6 in 42 matched gastric cancers and normal mucosal tissues, meantime β-actin was served as a quantitative standard marker, followed by agarose gel electrophoresis. Results： Mitochondrial COⅠ and ND4 transcripts in gastric cancers were higher than those in normal mucosal tissues, P〈0.01. Transcriptional levels of mtDNA were negatively correlated with grades of histological differentiation of gastric cancers, ND4 and COⅠ expression levels were higher in intestine type of gastric cancers than in diffused type, P〈0.05. Conclusion： Gastric carcinogenesis needs elevated mitochondrial transcripts and proteins to provide tumor cells with energy and other materials to maintain its malignant biological phenotype and behavior.