肝细胞生长因子及C-Met的表达与宫颈癌浸润和转移的关系

The Correlation between Expression of the Hepatocyte Growth Factor and c-Met/hepatocyte Growth Factor Receptor and the Invasion and Metastasis of Uterine Cervix Cancer

目的:研究肝细胞生长因子(HGF)及其受体C-Met在宫颈癌组织中的表达,探讨二者与宫颈癌浸润和转移的关系。方法:采用RT-PCR检测36例宫颈癌组织手术标本及31例正常宫颈组织标本中HGFmRNA和C-MetmRNA的表达,并进行相对定量研究。结果:宫颈癌组织中C-Met的阳性表达率显著高于正常宫颈组织。宫颈癌组织中,淋巴结转移组C-Met的表达水平高于未转移组;临床分期Ⅱ期组的表达水平高于ⅠB期组;中、低分化组的表达水平高于高分化组。结论:宫颈癌组织中存在C-Met的高表达,肿瘤细胞丰富的C-Met受体可能是通过与旁分泌途径获得的HGF结合而在宫颈癌浸润和转移的过程中发挥重要作用。

Objective： To investigate the expression level of the hepatocyte growth factor （HGF） and the c-Met/hepatocyte growth factor receptor mRNA in invasive cervical carcinoma and their associations with invasion and metastasis of cervical carcinoma. Methods： Expressions of the HGF mRNA and C-Met mRNA in postoperative samples of 36 cases with uterine cervix cancer and tissular samples of 31 cases with normal uterine cervix were determined using RT-PCR, and relative quantitation study was conducted. Results： The rate of positive c-Met mRNA expression in cervical carcinoma was significantly higher than that in normal uterine cervix. In primary tumors with the c-Met mRNA expression, the level in the group of lymphatic metastasis and of non-lymphatic metastasis was 1.6515±0.0850 and 1.2216±0.1191 respectively （P〈0.001）; the level in the group with stage I B and stage Ⅱ was 1.2086± 0.1387 and 1.5497±0.1802 （P〈0.001）, and the level in the group with the low and high grade was 1.4376±0.2387 and 1.2197±0.1419, respectively （P〈0.05）. Conclusions： There is a high expression of c-Met mRNA in the group of invasive uterine cervical cancer, and the HGF/c-Met pathway may play an important role in the invasion and metastasis of cervical cancer.