顺铂、氧化苦参碱对肝癌QGY细胞生长及端粒酶活性的影响

Effects of cisplatin,oxymatrine on the cell growth and telomerase activity of QGY hepatoma cells

目的:观察顺铂、氧化苦参碱对人肝癌细胞株QGY端粒酶活性的影响,进一步探讨两种药物的可能作用机制。方法:采用人肝癌细胞株QGY作为研究对象,MTT法检测不同浓度和作用时间的药物对细胞增殖的抑制作用,以及对QGY细胞粘附能力的影响;流式细胞仪分析细胞周期的分布及凋亡;PCR-ELISA法检测端粒酶活性的变化。结果:两种药物均具有抗QGY细胞增殖、阻滞细胞周期、诱导凋亡及抑制端粒酶活性的作用。结论:对端粒酶活性的抑制可能是两种药物发挥抗肿瘤作用的机制之一。

Objective：To investigate the anti-cancer mechanism of cisplatin, oxymatrine by observing their effects on telomerase activity. Methods：The inhibition of proliferation in QGY cells were estimated by MTT-test. Distribution of cell cycle and apoptosis were analysed using flow cytometry, The changes of telomerase activity were detected by PCR-ELISA method, Results：These two drugs could inhibit the proliferation of QGY cells, block the cell cycle, induce its apoptosis and inhibit the activity of telomerase.Conclusion： The inhibition of telomerase activity may be one of the mechanisms for the anti-cancer effect of the two drugs.