枸杞多糖对酒精性脂肪肝大鼠肝细胞色素P4502E1基因及蛋白表达的影响

Effects of lycium barbarum polysaccharide on the expression of hepatocyte cytochrome P4502E1 in rats with alcoholic fatty liver

目的:通过观察枸杞多糖(LBP)对酒精性脂肪肝(AFL)大鼠肝细胞色素P4502E1基因和蛋白的影响,揭示其治疗机制。方法:用乙醇灌胃10周造成大鼠慢性AFL模型,再用5%、10%LBP和10%阿托莫兰(GSH)治疗10周,戒酒组为阳性对照,作治疗前后比较:肝脏病理形态,肝匀浆还原型谷光甘肽GSH、丙二醛MDA含量,肝细胞色素P4502E1(CYP2E1)基因及蛋白表达水平。结果:LBP能明显改善酒精所致大鼠肝脏脂肪性病理变化,增加GSH含量,减少MDA产生,抑制CYP2E1基因及蛋白表达水平,与阿托莫兰组无显著差异,优于单纯戒酒组。结论:LBP抑制CYP2E1基因及蛋白表达,减轻脂质过氧化作用,有效的治疗AFL。

Objective：To study the effect of Lycium Barbarum Pclysaccharide （LBP）on the expression of hepatocyte cytochrome P4502E 1 （CYP2E 1） in rat model of alcoholic fatty liver （AFL）.Methods： The AFL rats models were established by ethanol intragastric infusion in ten weeks, then, 5% and 10% LBP and 10% GSH intragastric infusions were adopted to treat those AFL model rats. Liver pathologic changes were observed and CYP2E1 gene and protein expressions as well as the contents of malondialdehyde（MDA） and glutathione（GSH） in liver were detected and compared with those in the control group, treatment groups and abstinence treatment group. Results： Fatty degeneration in liver recovered normally in the LBP-treated groups. LBP could inhibit the expressions of CYP2E1 gene and protein, reduce the content of MDA, and increase the content of GSH. The curative effect of LBP was of no significant difference compared with GSH, but had more effect than abstinence treatment. Conclusion： LBP can prevent AFL though inhibiting the hepatocyte CYP2E1 expression markedly.