纳米TiO_2光诱导杀伤Bel-7402人体肝癌细胞研究

Damaging Effects of Nanosized TiO_2 on Bel-7402 Human Liver Cancer Cell Under Photoinduce

在光诱导条件下,采用HE染色法和四甲基偶氮唑蓝比色法(MTT法),研究了纳米TiO_2对Bel-7402人体肝癌细胞的杀伤作用,考察了在不同纳米TiO_2浓度、不同光照时间下纳米TiO_2的抑瘤效果,并且对抑瘤机制进行了探讨．结果发现：在光诱导条件下,适宜的了TiO_2浓度具有较高的抑瘤率,同时抑瘤过程表现出类似一级反应的动力学规律；在光诱导条件下,纳米TiO_2产生的活性氧组分与癌细胞膜内外的生物大分子反应,引起广泛的细胞结构破坏；造成癌细胞内Ca^(2+)离子稳态失去平衡；引发细胞微管相关蛋白2(MAP-2)表达的变化,促进微管发生重组,从而导致细胞凋亡和坏死．

The damaging effects of nanosized TiO2 on human Bel-7402 liver cancer cell were investigated by means of HE dye method and MTT colorimetric assay under condition of photoinduce. The influences of nanosized TiO2 content, and irradiation time on cancer inhibition were systematically studied, and the inhibition mechanism was primarily discussed. The results show that nanosized TiO2 exhibits good inhibition effects under appropriate TiO2 concentration, and the inhibition process obeys first order reaction rule approximately; In addition, activity oxygen produced by nanosized TiO2 under illumination can react with organicmolecules in and out the cancer cell membrane, so that lead to damage cell structure, result into imbalance of Ca^2＋ in the cancer cell, and induce microtubule reassembly due to expression changes of microtubule-associated protein-2, which are responsible for the apoptotic and dead cancer cells.