无机诱导因子复合性骨支架材料的生物相容学研究——兼论人体外复合细胞相容性观察

Investigation on the biocompatibility of mineralized induced grafts in bone tissue engineering

目的用预制型无机诱导因子复合性骨组织工程支架材料对实验动物山羊的下颌骨角部大型箱状缺损行骨重建,评价其及其降解产物对血液和植入区周围组织的生物相容性反应;和人体外复合细胞培养的生物相容性观察。方法15只雌性山羊作为实验组,术后4,8,12w处死动物对相关重要脏器进行大体解剖学和组织病理观察;另取雌性山羊15只作为对照组;培养人骨髓基质细胞行细胞形态、电镜和MTT观察。结果血液学检测,实验组与对照组均在正常值范围内,两组间差别无统计学意义;实验组重要脏器组织病理学未见形态改变和炎性细胞浸润;支架置入区域病检符合组织工程骨的愈合过程;人体外复合细胞培养的检测中未见明显异常。结论生物相容性观察提示:本支架材料无急慢性毒性反应和超敏反应,对人骨髓基质细胞的形态、生长和增殖无明显抑制作用,具有良好的生物相容性。

Objective The aim of this investigation was to observe the biocompatibility of mineralized induced grafts in experimental goats＇ mandible angles, as well as its degradation products＇ reaction to the blood and the tissue around embedding area, and to investigate the cytobiocompatibility in vitro of BMCs on mineralized induced grafts. Methods Fifteen female goats were used as experimental group. After 4,8,12 weeks, the goats were sacrificed and gross anatomy check and histopathological observation were taken on the relevant important viscera, and other 15 female goats were used as the control group. The culture BMCs in vitro were investigated by means of microphotograph and ECM and MTT assay. Results The results showed that the hematological outcomes both in the experiment group and control group were in the range of normal value, and the difference between the two groups had no statistical significance. In the experiment group, the histopathology of the important viscera had not showed any change and infiltration of inflammatory cells. The pathologyical examination in the area, the grafts put into the restorative processs of tissue engineering bone, No apparent difference was observed in BMCs cultured in vitro. Conclusion The biocompatibility observation indicated that this mineralized induced grafts had no acute or chronic toxicity reaction, no hypersensitivity, which had good biocompatibility.